HYALURONIDASE INHIBITORY ACTIVITY OF CHONDROITIN SULFATE AND GLUCOSAMINE SULFATE

Man Kyu Huh*

ABSTRACT

The hyaluronic acid, glucosamine, chondroitin are now used as health food by many persons in many countries including Korea. Hyaluronic acid (HA) is an important component of articular cartilage. Hyaluronidases are a family of enzymes that degrade hyaluronic acid. The inhibitory effects of HAase by chondroitin sulfate and glucosamine sulfate were assayed using a Morgan microplate assay. Methanol extract of the chondroitin sulfate was evaluated 36.0% on 800 mg/L and that of the ethanol extract was 35.1% at same concentration. Overall, compounds including glucosamine sulfate and chondroitin sulfate were slightly higher in HAase inhibitory activity than those of pure chondroitin sulfate. Methanol extract of the glucosamine sulfate with chondroitin sulfate was evaluated 41.7% on 800 mg/L and that of the ethanol extract was 40.8% at same concentration. There was significant difference between pure chondroitin sulfate groups and compounds including glucosamine sulfate and chondroitin sulfate groups (p <0.05). The results of this study suggest that the stem extract of chondroitin sulfate has significant HAase inhibitory effect. Chondroitin sulfate and glucosamine sulfate may be useful compounds for studies connected with the hyaluronidase inhibition.

Keywords: Chondroitin sulfate, Glucosamine sulfate, HAase inhibition, Hyaluronidase.