DETERMINE THE PREVENTIVE EFFECT OF POMETONE (POMEGRANATE SEED OIL) ON CARDIAC FUNCTION IN METHIONINE OVERLOAD IN TREATED FEMALE RABBITS

L.N. Abdulla*, Doaa A. Abdulwahab and Dina A.A Abdullah

ABSTRACT

This study was designed to investigate the role of pomegranate seed oil as antioxidant in ameliorating the deleterious effects of methionine overload on cardiovascular function of adult female rabbits. Thirty-Two female rabbits were randomly assigned into four equal groups (eight animals in each group) and treated for 42 days daily as follows: The first group were drenched corn oil, serving as control (group C), the second group (group T1) were intubated orally with methionine 100mg/kg. B.W, while thethird groups (groupT2) were intubated orally with methionine 100mg/kg. B.Wand pomegranate seed oil 30 mg /Kg. B.W, while theanimals in the groupT3were intubated orally with pomegranate seed oil 30 mg /Kg. B.W. The electrocardiographs (ECG) were recorded for rabbits in allexperimental groups at the same interval of the experiment, The analysis of ECG in rabbits treated with methionine (T1) showed a significant decrease in p wave, QRS wave and T wave amplitude, also significant increase in QRS and T wave interval as well as significant prolongation in P-Q and Q-T interval. On the other hand, the cardioprotective role of pomegranate seed oil was clarifiedin animals ofgroupT3, but it was less effective in methionine treated rabbits, where the oil fail to restore P, P-Q, Q-T wave in group T2 to normal level. In conclusion, the results of this study clarified the deleterious effect of methionine overload on electrical conduction of the heart represented by abnormality of ECG component, as well as the present study confirms the cardioprotective role of pomegranate seed oil as antioxidant by ameliorating effect of methionine and keeping the normal shape of QRS wave and prevent prolongation of Q-T interval.

Keywords: Pomegranate seed oil, Methionin overload, Antioxidant, oxidativee stress, cardiac muscle.