VALIDATED RP – HPLC METHOD FOR THE QUANTIFICATION OF ACE INHIBITOR PERINDOPRIL ARGININE
*Sk Manirul Haque and Elaref Ratemi
A new high performance liquid chromatography (HPLC) method was developed and validated for determination of
Perindopril Arginine (PA) in pharmaceutical formulations. Optimum separation was achieved in 12 minutes using
C18 Hypersil BDS column and elution was accomplished using mobile phase with flow rate of 1 ml/min. A linear
relationship between peak area and concentration of PA was observed in the range of 1 – 25 μg/ml. Precision and
accuracy of the method have been established according to the current ICH guidelines. The developed method was
successfully applied for the determination of PA in pharmaceutical formulations.
Keywords: Perindopril Arginine; RP-HPLC; Validation; Column; ICH; Pharmaceutical Formulations.
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