EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database ,

ISSN 2394-3211

Impact Factor: 6.222

ICV - 79.57

Abstract

DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPLC METHOD FOR ESTIMATION OF AMIODARONE HYDROCHLORIDE

Santosh V. Gandhi*, Pooja R. Alli and Rupali S. More

ABSTRACT

The aim of the present study was to develop a validated stability indicating reverse phase high performance liquid chromatography (RP-HPLC) method for estimation of Amiodarone hydrochloride. An isocratic, RP-HPLC method was developed using Hi Q SIL C18 (4.6 mm x 250 mm, 5 Î¼m) column, Acetonitrile and 0.5% formic acid (80:20 v/v) as mobile phase at flow rate of 1.0 ml/min. Detection and Quantification was carried at wavelength of 242 nm. The retention time (RT) of drug was 8.329 Â± 0.412 min. The method was validated with respect to linearity, precision, accuracy and robustness. The data of linear regression analysis indicated a good linear relationship over the range of 5-30 Î¼g/ml concentrations with a correlation coefficient (R2) of 0.9993. Amiodarone hydrochloride was subjected to different stress testing conditions. The degradation products were well resolved from the drug under the tested conditions.The developed method was found to be simple, sensitive, selective, accurate and precise for analysis of Amiodarone hydrochloride and can be adopted for routine analysis of drug in bulk and pharmaceutical dosage form.

Keywords: High performance liquid chromatography (HPLC), Amiodarone hydrochloride, Stability indicating method, Validation.

[Full Text Article]

SJIF Impact Factor: 6.222

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Its Our pleasure to inform you that, EJPMR 1 September 2023 Issue has been Published, Kindly check it on https://www.ejpmr.com/issue

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