EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

OUTBREAK OF NON-TUBERCULOUS MYCOBACTERIAL INFECTION IN SURGICAL WARD OF A TERTIARY CARE HOSPITAL

*Dr. Rajdeep Saha, MD, Dr. Apoorbaa Roy, Dr. Dipika Menon Mukherjee and Dr. Rajyasri Guha Thakurta, MD

ABSTRACT

Mycobacterium abscessus (M. abscessus) is a rapidly growing mycobacterial species which are ubiquitous in the environment and cause widespread infections. The incidence of these infections increased in recent decades and is of particular public health concern as nosocomial pathogens. This study was conducted to isolate and identify Non-Tuberculous Mycobacteria (NTM) from wound swab sample from post-operative surgical site infection along with finding out the source of infection and forming a treatment protocol for prevention of the same. 411 samples were collected. These samples were subjected to Gram Staining, Ziehl-Neelsen Staining and inoculating on culture plates containing Blood Agar, MacConkey Agar and Lowenstein-Jensen Media. The ZN stain done from the growth as well as the direct swab sample collected from the patients portrayed the presence of Acid Fast Bacilli. The isolated strains were further confirmed by PCR. Swab from OT instruments, trolleys, surgical gowns and other environmental samples and water from the boiler and copper drum was taken for determining the source of infection. Here too the isolates were sent for PCR for identification. 332 out of 411 patients were suffering from NTM infections. The PCR result confirmed it to be Mycobacterium abscessus. The samples collected to determine the source of infection revealed that the infection was due to inadequate sterilisation of boiler water which contaminated the surgical site. Disinfection protocol and antibiotic stewardship was prepared after several meetings. The patients were cured using Clarithromycin 500mg BD for 6 months.

Keywords: Mycobacterium abscessus, Surgical site infection, PCR, Antibiotic stewardship.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )