METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF NEBIVOLOL AND S – AMLODIPINE BY RP-HPLC METHOD

Abrar*, Srikanth Lingala, Nuzhath Unisa, Chandramouli Golla, Mallikarjun Bere and Naveen Chandra Kotagiri

ABSTRACT

The main object of analytical chemistry is to develop scientifically substantiated methods that allow the qualitative and quantitative evaluation of materials with certain accuracy. A rapid, simple and precise HPLC method was developed for simultaneous estimation of two drugs Nebivolol and S – Amlodipine from pharmaceutical dosage forms. The estimation was carried out using Sunfire C18 (4.6×250mm, 5μ) column; mobile phase consisting of Acetonitrile: Water (40:60 v/v); the flow rate of 0.9mL/min and ultraviolet detection at 220nm. Both the drugs were properly resolved having runtime of 6 min. the method was validated as a final verification of method development with respect to Precision, Linearity, Accuracy, Ruggedness and Robustness. The validated method was successfully applied to the commercially available pharmaceutical dosage forms, yielding very good and reproducible results.

Keywords: Nebivolol, S – Amlodipine, HPLC, Method development and Validation.