( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , Academia , 

 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57



Srivijay R.*, Vijayamirtharaj R. and Senthil Kumar N.


In pharmaceutical dosage forms, a new UHPLC method was developed and validated to simultaneously estimate Diclofenac Potassium and Dicyclomine Hydrochloride. By utilizing UHPLC, this method fills a gap in the existing literature. Optimizing various parameters of the chromatographic process was key to developing an effective method for separating and detecting drugs. International guidelines and regulatory requirements were followed for the validation of the method, including specificity, linearity, accuracy, precision, robustness, and suitability for the system. For the injection and analysis of mixed solutions containing Dicyclomine HCl and Diclofenac potassium, a Chromatographic separation was achieved on a Kromasil C18 (4.6 mm × 250 mm), 5μm.a mobile phase ratio consisting of (35:65) Water: Methanol at flow rate 1.0 ml/min, and total run time 8min, The injection volume 20μl. The detection wavelength is 263 nm. Approximately 2000 plates were found, indicating a successful chromatographic separation. With a tailing factor of less than 2 and a well-resolved peak, the peaks appear symmetrical and well-resolved. In order to ensure there were no interfering peaks, the retention times for Dicyclomine Hydrochloride and Diclofenac Potassium were found to be 2.745 min and 3.341min.As for Dicyclomine Hydrochloride, the correlation coefficient (R2) is 0.9999 and Diclofenac Potassium is 0.9999. This indicates a good linear relationship between drug concentrations and peak areas based on the high correlation coefficients. For Dicyclomine Hydrochloride and Diclofenac Potassium, the %RSD values were 0.87% and 1.08%, respectively, below the acceptable limit of 2%. As a result, the method is accurate and repeatable. In the study of Dicyclomine Hydrochloride, the mean percent recovery was 98.37%, while in the study of Diclofenac Potassium, the mean percent recovery was 98.31%, while the mean percent recovery was 100.34%.Under varied conditions, both Dicyclomine Hydrochloride and Diclofenac Potassium showed %RSD values within the acceptable range of 2%, demonstrating the robustness and reliability of the method. This study concluded that valuable insight is provided into the use of validated UHPLC methods in this study, which contributes significantly to the evolution of pharmaceutical analytical techniques.

Keywords: Diclofenac Potassium, Dicyclomine Hydrochloride, UHPLC, Chromatography, Method validation.

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  • New Issue Published

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia