SURPLUS THE BIOTIC RESISTANCE OF ANTIBIOTIC DRUGS WHEN CONJUGATED WITH SILVER FORMING NANO-COMPOSITES

Mohit Sharma*, Renu Sharma and Pardeep

ABSTRACT

Antibiotic resistant now a days is a cause of severe worldwide peril. To reduce the mortality along with infection rates a variety of approaches are now being familiarized. Blending of metals with antibiotics are operative against drug resistant microorganisms. Metallic nanoparticles (NPs) are reported to show antimicrobial activity. Herein, describe successful synthesis of ciprofloxacin HCl loaded silver nanoparticles (AgNPs) by co-precipitation method and their incorporation in the gel base of Carbapol 940. Successful synthesis of silver nanoparticles and their conjugation with ciprofloxacin HCl was confirmed by various test (zeta poteinal, particle size measurement, silver content, drug loading and entrapment efficiency). AgNPs were small in size with particle size distribution as obtained from TEM. To determine the release mechanism of formulated gel various models such as zero-order, first-order, Higuchi and Korsmeyer-Peppas were fitted to the release data. The improvement in antimicrobial upshot of drug was seen when combined with silver nanoparticles. Synergistic and additive effects were found with activity of ciprofloxacin HCl when doped with silver nanoparticles with in a gel base against S. aurease and MRSA During evaluation of minimum inhibitory concentration (MIC) values, similar concentration of prepared gel was capable of producing antibacterial activity.

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