FORMULATION AND EVALUATION OF 3D INJECTABLE BIODEGRADABLE HYDROGEL FOR THE TREATMENT OF PERIODONTAL DISEASES
Luay Thanoon Younis*, Tommy Julianto Bustami, Tara Bai Taiyeb Ali and Mohamed Ibrahim Abu Hassan
ABSTRACT
Periodontitis is an inflammatory disease resulting in the destruction and loss of tooth-supporting structures (periodontal ligament and alveolar bone). Objective: This study is designed to formulate, and evaluate a degradable bio-adhesive hydrogel contains titrated extract of Centella Asiatica (TECA 3%, w/w) for healing and regeneration of the periodontal cells in-vitro. Methods: The 3D potential hydrogel formulation is composed of pectin (P) and hydroxypropylmethyl cellulose (HPMC) in various concentrations. The hydrogels were formulated in the range of 1-5%, w/w of pectin and 2 - 4% w/w of HPMC. Characterization of the hydrogels was done using rheometer to study the effect of P and HPMC concentrations on hydrogel rheology behaviour. The texture of hydrogel was characterized using texture analyzer to evaluate its syringeability, firmness, adhesiveness and cohesiveness. Fibroblast proliferation assay was done to assess the effect of the gel on the cells proliferation. Southern blot assay was also done to ascertain the effect of the gel on maintaining fibroblast telomere length during inflammation. Results: The experiments showed that the hydrogels containing higher P and HPMC have potential properties to deliver active compound. Incorporation of TECA in the hydrogels formulations does not affect the physical properties of the hydrogel. One of the best hydrogel formulations with high content of pectin and HPMC were selected. Cell proliferation assay was done to assess the proliferative effects of TECA on the human periodontal ligament fibroblasts (PDLFs). The parameters depicted significant activity of TECA hydrogel in enhancing the proliferation of fibroblast together with preserving their telomere length during inflammation. Conclusion: Due to the regenerative effects of TECA hydrogel, and the suitable gel mechanical properties achieved, the formulated bioadhesive hydrogel may be used as an adjunct for the treatment of periodontal disease and regeneration of the periodontal structure.
Keywords: Hydrogel, periodontal disease, TECA, fibroblast, telomere length.
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