IDENTIFICATION OF ACTIVE COMPOUNDS USING GC–MS AND ANTIOXIDANT ACTIVITIES OF HIBISCUS CANNABINUS FLOWERS
N. Muniyappan and M. Sasikala*
ABSTRACT
Natural antioxidants present in the plants scavenge harmful free radicals from our body. Antioxidants exert their mode of action by suppressing the formation of reactive oxygen species either by inhibition of enzymes or by chelating trace elements. Free radicals are the chemical species that have an unpaired electron and play very important role in human health and beneficial in combating against several diseases like cardiovascular disorders, lung damage, inflammation etc. These can damage cell membranes and other vital cell components, such as genetic material in the cell nucleus, and can inactivate enzymes. Damage to body cells and molecules by oxygen containing free radicals has been implicated in a wide variety of diseases. Antioxidants in different parts of plants such as ascorbic acid, vitamin E and phenol compounds possess the ability to reduce the oxidative damage associated with many diseases including cancer, cardiovascular diseases, cataracts, atherosclerosis, diabetes, arthritis, immune deficiency diseases and ageing. This study was aimed to evaluate in vitro antioxidant activities of Hibiscus cannabinus flowers. For in vitro antioxidant screening, DPPH, ABTS, hydroxyl radical scavenging, hydrogen peroxide scavenging activities and ferric reducing power models were used in this research. Also, the current work, analyses the chemical constituents Hibiscus cannabinus flowers using GC-MS.
Keywords: Hibiscus cannabinus flowers, GCMS, Antioxidant, DPPH, ABTS
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