FORMULATION AND CHARACTERISATION OF CHITOSAN BASED LAMIVUDINE NANOPARTICLES

P. Pravalika Reddy*, Dr. B. Madhava Reddy, Dr. D. Jaya Prakash, Dr. K. Latha and Dr. D. Prashanthi.

ABSTRACT

The aim of the present work is to formulate and characterize nanoparticles of lamivudine drug. Lamivudine is an antiretroviral drug and BCS Class-III having high solubility and low Permeability. Nanoparticles of Lamivudine are fabricated by using chitosan polymer and pregelated Sodium alginate by Ionotropic pregelation method and optimization in terms of polymer concentration, cross‐linking agent,stirring time and stirring speed. Calcium chloride is also included in the formulation for pregelation of sodium alginate. Different formulations of nanoparticles are prepared using different concentrations of chitosan, Sodium alginate, stirring time and speed. The drug loaded optimized lamivudine nanoparticles showed average particle size of 77.7 nm. Drug entrapment ranged between 67.79%‐ 89.98%. Invitro release studies revealed that the rate of drug release from NP 2 Formulation is 89.55% in 24 hours. Release of drug followed first order and the mechanism is super case II transport, indicates swelling of polymer with diffusion.

Keywords: Lamivudine, Nanoparticles, Chitosan, Sodium alginate, Calcium chloride, Ionotropic Pre gelation Method.