EVALUATION OF ALPHA-AMYLASE INHIBITORY ACTIVITY OF GREWIA ABUTILIFOLIA LEAVES

Abdullah-Al-Mamun*, Md. Shariful Islam, Md. Ibrahim Sunny, Md Abdur Rahaman Miah, Umma Hafsa, Shakila Akter Zabin, Pankoj Chandra Roy 

ABSTRACT

Alpha-amylase inhibitors are oral anti-diabetic drugs used for diabetes mellitus type 2 that work by preventing the digestion of carbohydrates (such as starch and table sugar).Inhibition of Alpha-amylase enzyme that plays a role in digestion of starch and glycogen is considered a strategy for the treatment of disorders in carbohydrate uptake such as diabetes and obesity.Plants are an important source of chemical constituents with potential for inhibition of Alpha-amylase and can be used as therapeutic or functional food sources. Grewia abutilifolia belonging to the family Tiliaceae,is a tree and that is used in traditional medicine for the treatment of diabetic mellitus. In the present study, methanolic extracts of Grewia abutilifolia leaves which are used in the Ayurvedic traditional system of medicine to treat diabetes were tested for their inhibitory effect on α-amylase. The crude methanol extracts (6.88 ± 0.14) of Greiwa abutilifilia with pet-ether fraction, (21.13 ± 25.24), chloroform fraction(6.02 ± 1.15) and methanol+water fraction (5.2± 0.80). Among all fractions acarbose & methanol+water showed highest percentage inhibition & the Ic50 value was 5.7 ± 0.24 & 5.2 ± 0.80. So, methanol + water extracts has the highest percentage of inhibition than acarbose. As a results, Grewia abutilifolia leaves properly inhibits the alpha-amylase in diabetes.

Keywords: Alpha-amylase, Carbohydrate Metabolism, Diabetes Mellitus, Grewia abutilifolia.