SYNTHESIS OF COPPER NANOPARTICLES FROM BEE PROPOLIS EXTRACT AND ITS ANTIBACTERIAL PROPERTY

*Shubharani Ramnath

ABSTRACT

Nature has diverse processes for synthesis of nanoscaled inorganic materials which have contributed to the enhancement of innovative area of research based on biosynthesis of nanoparticles. In the present study, copper nanoparticles were synthesised by using simple method of chemical reduction from ethanol extract of propolis. The biosynthesized copper nanoparticles were characterized with UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The UV-Vis spectra confirmed the peak for synthesized copper nanoparticles at 448 nm. The XRD analysis showed that the copper nanoparticles are crystalline in nature. The FTIR measurement was carried out to identify the possible bio molecules responsible for efficient stabilization of copper nanoparticles. These biologically synthesized copper nanoparticles were effectively utilized for antibacterial activity against human pathogens viz, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Strptococus mutans, Bacillus subtilis, Klebsella pneumonia, Enterococcus faecalis and Citrobacter spp. The antibacterial activity was tested by well diffusion zone inhibition method. Staphylococcus mutans, Klebsella pneumonia and Enterococcus faecalis exhibited the highest zone of inhibition in copper nanoparticles.

Keywords: Antibacterial, biosynthesis, copper nanoparticles, propolis.