ESTIMATION OF PARACETAMOL, ASPIRIN, IBUPROFEN, CODEINE AND CAFFEINE IN SOME FORMULATED COMMERCIAL DOSAGE USING UV – SPECTROSCOPIC METHOD.

Ahmed Mahdi Saeed* and Noor Qasim Ahmed

ABSTRACT

The estimation of paracetamol (PCM), aspirin (ASP), ibuprofen (IB), codeine (COD) and caffeine (CAF) drugs percentage in standard and some formulated commercial dosage using UV-Spectroscopic method was investigated in this work. The absorption maxima of drugs were found to be at (244.8 nm, 238 nm, 220.6 nm, 278 nm and 272.6 nm) for PCM, ASP, IB, COD and CAF respectively in deionized water: acetonitrile (90:10 v/v) as solvent. These wavelengths were selected for the analysis of drugs as standard and formulated samples. The purposed method obeyed Beer-Lambert's Law in the concentration range of (0.4-40 μg/mL) for PCM, (0.5-100 μg/mL) for ASP, (0.4-40 μg/mL) for IB, (1-100 μg/mL) for COD and (0.2-20 μg/mL) for CAF with R2 values of (0.9994, 0.9989,0.9992, 0.9995 and 0.9979) for PCM, ASP, IB, COD and CAF respectively. Recovery means were found to be (100.05, 102.20, 102.88, 100.17, and 99.95) for PCM, ASP, IB, COD and CAF. The method was applied for the estimation of the active gradient of the drugs in different samples of formulated dosage. The accuracy of method was validated by mean percentage recovery which was found to be in the acceptable range.

Keywords: Estimation, UV- Spectroscopic, Formulated, Recovery.