EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

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Abstract

CONTENT OF TOTAL PHENOLIC COMPOUNDS AND ANTIOXIDANT POTENTIAL OF ORIENTAL TOBACCO VARIETIES (NICOTIANA TABACUM L.)

Ana Cvetanovska*, Marija Krstic and Lenka Cvetanovska

ABSTRACT

During 2011 and 2012, experimental plots were set with oriental tobacco type Prilep (variety P-66), type Јaka (variety ЈK-125/3) and type Basma (variety Basma 82) on the experimental field at the Scientific Institute in Prilep. The research was conducted in order to analyze the selected oriental varieties of tobacco, by monitoring the parameters that determine their high antioxidant capacity. The antioxidant potential of the tobacco was measured by determining the content of total phenolic compounds, scavenging potential on DPPH (1,1-diphenyl- picrylhydrazyl), reducing ability on Cooper (II) neocuproine (CUPRAC method) and the activity of catalase. The results showed positive correlation between the content of phenolic compounds and the scavenging potential of the methanolic extract on both DPPH and Cu2+ radicals. The highest concentration of phenolic compounds was detected in Јaka variety harvested in 2011 (28,10± 0,48 mg/g), in which we also recorded the highest scavenging activity of the methanolic extract on the DPPH radical (78 ± 2,20%), the CUPRAC (183,99 ± 2,70 μM TE/g DW) and highest catalase activity (8,96 ± 0,11 mg decomposed H2O2). The results showed that the methanolic extracts from Јaka–125/3 have the highest antioxidant activity among the tested varieties.

Keywords: Nicotiana tabacum L.; antioxidant activity; DPPH; CUPRAC; catalase activity.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia